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BillsFanNC

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    The Waiting Game

    in Politics, Polls, and Pundits

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    1 hour ago, Wacka said:

    That is an animation. but about 25 years ago , where I worked  was pioneering that kind of stuff. We labeled bacteria , and tumor cells with luciferase (the enzymemakes fireflies glow)  and inject them into mice.  Then later inject the me with luciferin (the molecule that is cleaved to produce light)knock them out and image the light with  a very powerful camera.  Some people were making transgenic mice that had different gene switcheds(promoters) on the luciferase gene so you could see where those genes were turned on in the development of the mouse. I personally worked on making the yeast Candida albicans) that causes yeast infections glow.  That yeast has a different code for one amino acid , so I had to change the code for that amino acid in the luciferin gene (8 places) so it would work in that yeast. Had a bunch of cloning to do that took  several moths to do. The pictures looked like the end of that animation.

     

    I was injecting I-125 labeled protein and tracking signal in different tissues. IV vs. IP vs subQ injections.

     

    This was also back in the dark ages where you could iodinate your own proteins/peptides. I think the labeling chemistry was at tyrosine residues if memory serves.

     

    After the labeling you had to go get a thyroid scan to make sure no free radioactive iodine made it there. 

     

    Fun times.

     

    Years later I worked on developing a chemiluminescent assay that used the luciferin/luciferase chemistry.

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